| Volume: 8; Issue: 2; December 2026 |
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| Title : DOSE-DEPENDENT NEUROPROTECTIVE EFFECTS OF RESVERATROL AND GSPE AGAINST COLD STRESS-INDUCED OXIDATIVE DAMAGE IN RAT BRAIN REGIONS |
| Authour(s): D.M. Tilak and P.M. Basha |
| Keywords: Cold stress, Grape-seed extract, Hippocampus, Lipid, Neuroprotection, Resveratrol. |
| Abstract: Chronic cold stress induces oxidative neurotoxicity through sustained HPA axis activation. The present study evaluated the duration-dependent effects of cold stress and the dose-optimized neuroprotective potential of polyphenols in Wistar rats. Animals were exposed to cold stress (5°C, 6 h/day) for 30 days, and LPO, MnSOD and serum cortisol levels were assessed in optimal exposure duration. A 15- day protocol produced significant oxidative stress with pronounced hippocampal vulnerability (p< 0.05) and no mortality. Trans-resveratrol and grape seed proanthocyanidin extract (GSPE) were administered at 25-100 mg/kg, individually and in combination. FTIR and UV-visible spectroscopy confirmed compound identities resveratrol (O–H stretching at 3200-3500 cm⁻¹; λ_max 215 and 305 nm) GSPE (λ_max ~280 nm). Cold stress increased LPO and cortisol in a duration dependent manner and reduced MnSOD activity, indicating impaired antioxidant defense. Polyphenol treatment showed a dose-dependent neuroprotective response, with maximal efficacy at 75 mg/kg. Higher doses (100 mg/kg) showed reduced effectiveness, consistent with hormesis. Combination treatment (50 mg/kg each) restored MnSOD activity and reduced LPO more than higher individual doses, indicating a synergy. These findings show moderate-dose polyphenol combinations attenuate cold stress–induced oxidative neurotoxicity validate a 15-day model for investigating stress-related neurodegenerative mechanisms. |
| DOI: https://doi.org/10.46505/IJBI.2026.8207 |
| How to cite this article: Tilak D.M. and Basha P.M. (2026). Dose-dependent Neuroprotective effects of resveratrol and GSPE against cold stress-induced oxidative damage in Rat brain regions. International Journal of Biological Innovations. 8(2): 207-215. https://doi.org/10.46505/IJBI.2026.8207 |
Pages: 207-215 | Viewed: 18 | Downloaded: 15| Download: PDF (4453KB) |
